Animal Reproduction, Gynecology & Obstetrics

Newer
techniques of processing and freezing of goat semen which was otherwise poorly
freezable were evolved and commercialized. A centrifugation regime is
standardised and a commercial bovine semen extender (Optixcell) was used for
cryopreservation of goat semen and found effective as compared to Tris and
Bioxcell extender. Artificial inseminations are performed at field level using
cryopreserved semen and different doses of semen are given to ALDA.

Artificial
insemination allows small scale raiser to keep a few sows without maintaining a
boar; eliminates the problem of mating boars and sows of different sizes;
increases the number of sows bred by a boar and the possibility of extending
the boars’ productive life and reduces breeding cost. Fresh semen was collected
from Hampshire boar, processed and liquid semen was preserved at 15°C. Artificial
insemination was performed in swine using liquid boar semen in field level for
genetic improvement of the pig population of Assam.

Buffalo
semen were collected and cryopreserved using TRIS extender with glycerol and
artificial insemination was done successfully.

Due to faulty management cow used to suffer from
different metabolic or infectious diseases which results in infertility in cow.
Which affect the production and reproduction in cow with a negative impact on
the economy of the farmer. Various infertility cum treatment camps were
conducted in different districts of the state.Hormonal interventions viz.,
Ovsynch, Ovsynch plus, Double synch and Heatsynchand supplementation of feed
additives (mineral mixture, bypass protein, herbal anti stressor, selenium and
vitamin E) were efficaciously used to treat postpartum anestrus cow.

Some of the therapeutic protocols are explained below:

·       1)A total of 85 non- infectious repeat
breeding crossbred cows were fed with 30 gm mineral mixture per animal per
day for 30 days, 200 gm bypass fat per animal per day for 30 days, vitamin A, D
& E suspension 20 ml per animal per day for 15 days in locally available
feed followed by intramuscular administration of 15 ml meloxicam (a cox₂
inhibitor) on day 16 and 17 post AI. Out of 85 non- infectious repeat breeding
crossbred cows with supplement and meloxicam injection, 67 cows (78.82 %) were
considered to be pregnant on non-return basis and 31 (46.27 %) cows were found
to have conceived on 1^st A.I.

2) A total of 147 postpartum anestrus cows were supplemented with 30 g of Mineral
Mixture, 10 g  Bypass Protein and 10 ml
Selenium&Vitamin E for 20, 10 and 10 days respectively in concentrate feed
on 147 crossbred post-partum anoestrous cows in field condition.Out of 147 post-partum anoestrous cows
104 (70.75 %) responded to treatment of which 62 (59.62 %) did not return to
oestrus on first A.I

3) A total of 42 infectious
repeat breeder cows were treated with Lugol’s iodine (0.25%) solution along
with the supportive therapy which includes a) Bypass Fat (Fatomax, Intas
Pharmaceuticals Limited) @ 150gm per day per animal with concentrate mixture
for 20 days; b) Mineral mixture (Minfa Gold, Intas Pharmaceuticals Limited) @ 30gm
daily per animal with concentrate mixture for a period of 30 days; c) Injectable
Phosphorus (Tonophosphan Vet, MSD-Animal Health, Pune, India)2 gm
intramuscularly per day for three occasions at alternate days; d) Vitamin A
(Intavita H, Intas Pharmaceuticals limited) @ 5ml intramuscularly for three
occasions at alternate days which gives the best result in terms of conception.

For the first time
in India, 8 cell and 4 cell pig embryos were developed through in-vitro maturation and fertilization in
collaboration with ICAR RC for NEH Region, Umiam, Meghalaya.