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AICRP on Nematode, AAU, Jorhat

Academic Department

AICRP on Nematode, AAU, Jorhat

Constituent Department under Directorate of Research(Agri)

Assam Agricultural University (AAU), Jorhat a pioneer institute, has been working on identification and management of agriculturally important plant parasitic nematodes extensively through the Department of Nematology for decades. Initially the project was developed by Indian Agricultural Research Institute under the title “All India Co- ordinated Research Project on Nematode pests of crops and their Control” which was funded by the Department of Science and Technology, Govt of India for a period of two years. Subsequently, in 1979 the project was taken over by the ICAR for funding and continued under 6th Five Year plan. Dr.P.N.Phukan, joined as first Principal Investigator in 1979.

Department Faculty Members

2 Faculty

Associated Faculty

Dr. Debanand Das

Principal Scientist Associated Scientist View Profile

Dr. Nibedita Borgohain

Junior Scientist Associated Scientist View Profile

Department Achievements & Accolades

20 Records

Rice:

1. Management of rice root-knot nematode, Meloidogyne graminicola in direct seeded rice: Application of Pseudomonas fluorescens @ 20 g/m2 at sowing for the management of rice root-knot nematode, Meloidogyne graminicola in direct seeded rice.

Pulses:

1.Management of root-knot nematode, Meloidogyne incognita in mung: Seed treatment with Neem Seed Kernal Powder @ 5 g/kg and Trichoderma viride @ 5 g/kg separately for the management of root-knot nematode, Meloidogyne incognita in mung.

Fiber crops(Jute) :

Management of disease complex of Jute caused by Meloidogyne incognita and Ralstonia solanacearum: Application of Carbofuran @ 1 kg a.i./ha and Biofor pf @ 2.50 kg/ha (with vermicompost at 1:10) one day before sowing of seeds for the management of root-knot nematode, Meloidogyne incognita and Ralstonia solanacearum in jute.

Citrus:

Management of citrus nematode, Tylenchulus semipenetrans using bioagent: Apply 200 g Trichoderma viride per plant (enriched with vermicompost/FYM) for management of citrus nematode, Tylenchulus semipenetrans.

( Procedure for enrichment:20 kg Trichoderma viride mixed with 180 kg of Vermicompost / well decomposed FYM. This mixture should be covered with gunny bag and to be placed in shade for 15 days. Periodical sprinkling with water to be done in order to maintain adequate humidity. 200 g of this mixture should be applied per plant around the base twice in a year, along with application of fertilizer.)

· Avoidable loss due to Meloidogyne graminicolain rice is 16.52 -20.60 %

· Avoidable loss due to Hirschmanniella oryzae in rice is 10.23 - 13.08%

Avoidable loss due toMeloidogyne incognita Race-2in Tomato, Brinjal, Mung, Chilli, French Bean, Okra, Cucumber, Blackgram, Cowpea, Bitter Gourd, Pea and Chick Pea is 12.86-13.2%, 15.56-16.65%, 23.33 – 26.57%, 14.75%, 17.62-19.8%, 17.6-21.19%, 15.65-17.92%, 15.85%, 17.78%, 15.38%, 16.0% and 14.53% respectively

A
total of 321 germplasm of rice was screened in
vitro against Meloidogyne
graminicola during 2012 - 2023, out of which 08 germplasm ((IVT-2016-4,
IVT-2016-6, IVT-2016-9, IVT-2016-14, IVT-2016-17, IVT-2016-19, IVT-2016-38,
IVT-2016-42) were recorded resistant and 38
germplasm were recorded as moderately resistant ((AV-1-13-3702, AV-1-13-3708, AV-1-13-3710,
AV-1-13-3717, AV-1-13-3719, AV-1-13-3722, AV-1-13-3725, A1-14-04, A2-14-25, A2-14-28, GM-14-36, GM-14-37 , AB104, AB 108, IVT-2016-5, IVT-2016-8, IVT-2016-10,
IVT-2016-11, IVT-2016-12, IVT-2016-13, IVT-2016-15, IVT-2016-16, IVT-2016-18,
IVT-2016-20, IVT-2016-21, IVT-2016-25, IVT-2016-27, IVT-2016-28, IVT-2016-29, IVT-2016-30, IVT-2016-31,
IVT-2016-32, IVT-2016-33, IVT-2016-34, IVT-2016-35, IVT-2016-39, IVT-2016-40,
IVT-2016-41, IVT-2016-44) to M. graminicola.

1. Pseudomonus flourescens enriched FYM @ 500 kg/ha (1.0 kg Pf in 100 kg FYM incubated for 15 days) + soil application of carbofuran@ 1.0 kg a.i./ha at 45 DAS) was found effective in reducing population of Meloidogyne graminicola and increasing yield of direct seeded rice

2. In transplanted rice, maximum grain yield (3.715 t/ha), minimum soil and root population and RKI of M. graminicola were recorded when carbofuran was applied @ 1.0 kg a.i./ha at sowing

3. The combine application of neem cake @100g/m2 and carbofuran @1.0kg a.i./ha after 45 days of sowing was effective in reducing the population of M. graminicola and increasing yield in direct seeded ahu rice

4. Trichoderma viride @10g/kg seed as seed treatment and soil application @2.5 kg/ha at 45 DAS was found to be most effective in reducing population of M. graminicola(52.25%) and increasing yield (25.23%) with ICBR 1:5.25

5. Soil application of Pseudomonas fluorescens @ 20g/m2 and Bacillus subtilis @20g/m2at sowing were found effective against M. graminicola in direct seeded rice

6. Nursery treatment with Biofor pf-2 @20g/m2 was found effective in reducing population of M. graminicola and increasing yield

135
germplasm of Brinjal and 100 germplasm of Okra were screened against Meloidogyne incognita in controlled
condition. One each of brinjal (IC
112315)and okra germplasm (EC
306700) was recorded as resistant
while, three and two of brinjal and okra respectively were recorded as
moderately resistant.

1. Application of Purpureocillium lilacinum@ 20g/m²(cfu 2x10⁶) + neem cake @100g/m² was found effective against M .incognita in cowpea, where the yield was 51 q/ha as against 39 q/ha in untreated control

2. Seed treatment with Purpureocilliumlilacinum @ 2.5 ml/kg + Pochonia chlamydosporia @ 2.5 ml/kg followed by soil application of vermicompost @ 2.5t/ha enriched with P. chlamydosporia and P. lilacinum each @ 5ml/kg was found effective against M. incognita in okra where the yield was 8.81 t/ha as against 6.58 t/ha in untreated control

3.Biofumigants, cabbage leaves @ 50 t/ha was found effective in reducing population of M. incognita in okra

4. Nursery bed treatment with neem cake @ 2 t/ha is found effective in reducing infestation of root-knot nematode in main field and increasing yield

· Random survey was conducted in twelve districts of Assam for occurrence of Meloidogyne graminicola and Hirschmanniella oryzae.

Shillongoni (26⁰21.942ʹ; 092⁰38.447ʹ), Kuruabahi (26⁰18.603ʹ; 092⁰36.868ʹ), Bhalukamari (26⁰17.173ʹ; 092⁰33.054ʹ) Burhapahar (26.572032, 93.143783), Lanka (25.926938;92.955365 & 25.928298; 92.957468), Bechamari (26.407948; 92.47003)of Nagaon district; Areajhar (26⁰15.291ʹ; 090⁰24.133ʹ), Andutzar (26⁰25.112ʹ; 090⁰25.262ʹ), Kajaikata (26⁰11.134ʹ; 90⁰10.478ʹ) of Dhuburi district; Nayagaon (26⁰10.284ʹ; 90⁰11.04211) of Bongaigaon district wewre recorded as hot spot area for M. graminicola.

Ahom gaon(26.398686; 92.479761), Kuruabahi 26⁰18.603ʹ E-092⁰36.868ʹ) of Nagaon district; Tarajuli of Sonitpur (26⁰51.105ʹ; 92⁰38.023ʹ) was identified as hot spot of Hirschmanniella oryzae

· Four deep water rice growing districts of Assam were surveyed during 2012-22 for occurrence of ufra disease

Rantijan (27⁰07.578ʹ; 94⁰07.989ʹ) and Dolpata (27⁰05.430ʹ; 94⁰06.854ʹ) of Lakhimpur and Nanadi bhakatgaon (27⁰25.109ʹ; 94⁰23.281ʹ), Rangajan (27^o25.098ʹ; 94^o 23.268ʹ) of Dhemaji district were identified as hot spots of Ditylenchus angustus. However, from 2019 onwards infestation of Ditylenchus angustus in these district become drastically lo

· Random survey was conducted in eleven districts of Assam for occurrence of Meloidogyne incognita in vegetable crops.

Panibhoral(26⁰41.932ʹ; 93⁰08.976ʹ)and Dagaon (26⁰39.733ʹ; 93⁰09.766ʹ)of Sonitpur district; Bogolijan (27⁰11.922ʹ; 94⁰03.049ʹ), Nalkata (27⁰14.772ʹ; 94⁰04.817ʹ) and Gaurisali(27⁰14.869ʹ; 94⁰03.383) of Lakhimpur district; Satber (26⁰14.589ʹ; 90⁰14.362), Surajkhata (26⁰23.434ʹ; 090⁰ 22.065ʹ), Gutipara (26⁰10.284ʹ 090⁰11.042ʹ), Puran khambari (26.079197, 89.971010) and Hakama (26⁰31.951ʹ; 92⁰55.803ʹ) of Dhuburi district; Danichapori (26⁰44.677ʹ; 093⁰56.445ʹ), Nagajurigaon (26.6797; 93.4035), Kharua (26.4916; 93.1932) Anandapur (26.7075; 93.2085) of Golaghat district;Goroimari, Bihdiajajikona, Kamalpur, Karipara, Bamungaon, Ahatguri, Ratangaon, Patgaon of Kamrup district; Charigaon (26^o47.417ʹ; 094^O14.494ʹ),Upardeori (26⁰49.339ʹ;94⁰6.707ʹ),Namdeori(26⁰48.199ʹ;94⁰ 5.818ʹ)Allengmora (26⁰49.246ʹE094⁰1479ʹ) and Morangaon (26⁰61.708ʹ; 94⁰18.912ʹ) of Jorhat district; Puranigudam (26.366064; 92.785984), Ambagan (26.51774;92.85436); Lanka (25.928424; 92.95504); Simaluguri (26.362577; 92.643992) of Nagaon district were identified as hot spots of M. incognita in vegetables

Random survey was conducted in three pulse growing districts of Assam for the occurrence of Meloidogyne incognita.

Satber (26⁰14.589ʹ; 90⁰14.362) in Dhuburi district; Morangaon (26⁰61.708ʹ; 94⁰18.912ʹ) of Jorhat district; Niz Kothiatoli (26.211786; 92.725256), Uttar Chang Chaki (26.162369; 92.671628) of Nagaon district was identified as hot spot area of M. incognita in pulses

· Random survey was conducted in five Jute growing districts of Assam.

Dagaon (26⁰39.733ʹ; 93⁰09.766ʹ) of Sonitpur district, Tokoubari (26⁰21.601ʹ; 92⁰37.717ʹ), Lochanabari (26⁰16.023ʹ; 92⁰24.139ʹ) of Nagaon District; Gutipara(26⁰10.284ʹ;90⁰11.042ʹ), Islamnagar (26⁰12.564ʹ; 90⁰13.554ʹ) of Dhuburi district were recorded as hot spot area of M. incognita

· Random survey was conducted in eleven districts of Assam for the occurrence of plant parasitic nematodes in fruit crops (banana, citrus, guava, papaya).

· Major plant parasitic nematodes recorded around the rhizosphere of different fruit crops were: Meloidogyne incognita, Helicotylenchusdihystera, Hoplolaimus indicus, Tylenchorhynchusleviterminalis, Macroposthoniaspp.

Trichoidorusborai was recorded for the first time around the rhizosphere of banana

Steinernema abbasi, Oscheius spp, Heterorhabditis bacteriophora, Steinernema
ceratophorum, Steinernema ceratophorum, Steinernema tami, Steinernema spp. were isolated and identified from different districts of Assam

Study
on the host races of M. graminicola
revealed that based on Rf value, rice (PB1121and Century Patna), weeds (Dactyloctenium aegyptium and Leptochloa
chinensis) were found as host of M.
graminicola

200,
195, 210,81, 5, 4, 68 and 80 germplasm of blackgram, green gram, lentil, field
pea, rajmah, lathyrus, pigeon pea and chickpea, respectively were screened against M. incognita under controlled condition. One of greengram, 8 of
lentill, 4 of field pea were recorded resistant against the target nematode

1. Meloidogyne incognita in mung bean can be effectively managed by following mung – toria – paddy or Black gram-potato-maize rotation. However, in blackgram as main crop the sequence black gram – cabbage – okra is economically good

2. soil application of neem cake @1t/ha + seed treatment with B.subtilis @10g/kg seed was found to be effective in reducing the infestation of Meloidogyne incognita and increasing yield of mung bean

3. soil application of neem cake @1t/ha + seed treatment with B.subtilis @10g/kg seed was found to be effective in reducing the infestation of Meloidogyne incognita and increasing yield of cow pe

Disease complex of Jute (M.incognita
and Ralstonia
solanacearum) could be managed by
soil application of Trichoderma
viride @ 2.5 kg/ha at sowing the
seeds and can obtain more fibre yield (35.51%)

Trichoderma viride @20g/m2 was effective in declining the
population of Tylenchulus semipenetrans
in Assam lemon where 44.82 ton/ha yield was recorded, which was an increase of
20.38% over control

Bacillus altitudinis, B. subtilis, B. Cereus, B.marisflavi(
2 isolates),B. velezensis Microbacterium arborescens, Exigobacterium indicum, Stenotrophomonas
maltophila, Alcaligenes faecalis, Rhizobium pusense, Bacillus marisflavi ( 2 isolates), and
Pseudomonas spp were isolated and
tested in vitro for their efficacy
against M. incognita

1. Cucumber in protective cultivation can be protected from Meloidogyne incognita using bio control agents. Spot application (SA) of Bacillus pumilus @ 1kg/ polyhouse (1000 m2) at the time of planting is effective in managing RKN

2. Capsicum in protective cultivation can be protected from Meloidogyne incognita by Spot application of G. fasciculatum@ 1kg before planting + SA of Pochonia chlamydosporia @ 1kg/ polyhouse (1000 m2) at 45 DAP

Research & Extension Projects

1 Total Projects

Ongoing Projects (1)

ICAR Ongoing

AICRP ON AGRICULTURALLY IMPORTANT NEMATODES

Investigations on plant parasitic nematodes in Assam Agricultural University were initiated in 1970 in the Department of Botany and Plant Pathology. Dr.A.K.Roy was the pioneer worker who reported the occurrence of ufra nematode Ditrylenchus angustus and rice root knot nematode Meloidogyne graminicola in the state. In 1977, the Indian Agricultural Research Institute developed a project entitled “All India Co-ordinated Research Project on Nematode pests of crops and their Control” which was initially funded by the Department of Science and Technology, Govt of India for a period of two years. Out of ten centres in 1977, the Assam Agricultural University was selected as one of the centres with the position of one Nematologist, two Junior Nematologists, one Fieldman and one Laboratory Attendent. Subsequently, in 1979 the project was taken over by the ICAR for funding and continued under 6th Five Year plan. Dr.P.N.Phukan, after completion of his Ph.D.in Nematode Taxonomy from IARI joined in the Project as the Principal Investigator in 1979. The ICAR had changed the title of the project to-“ All India Co-ordinated Research Project with Integrated Approach for their Control” in 1987 and continuing till date.

Head of the Department (HOD)

AICRP on Nematode, AAU, Jorhat

aicrp.nematode[at]aau.ac.in

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