AICRP on Nematode, AAU, Jorhat

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AICRP on Nematode, AAU, Jorhat
Assam Agricultural University (AAU), Jorhat a pioneer institute, has been working on identification and management of   agriculturally important plant parasitic nematodes   extensively through the Department of Nematology for decades. Initially the  project was developed by Indian Agricultural Research Institute under the title “All India Co- ordinated Research Project on Nematode pests of crops and their Control”  which was funded by the Department of Science and Technology, Govt of India for a period of two years. Subsequently, in 1979 the project was taken over by the ICAR for funding and continued under 6th Five Year plan. Dr.P.N.Phukan, joined as first Principal Investigator in 1979. 
 
Projects
    • ICAR sponsored project on "AICRP ON AGRICULTURALLY IMPORTANT NEMATODES",

Our Faculties

Dr. Debanand Das

Principal Scientist (Associated Scientist)

Dr. Nibedita Borgohain

Junior Scientist (Associated Scientist)
Achivements
  • Rice:
    1. Management of rice root-knot nematode, 
    Meloidogyne graminicola in direct seeded rice:     Application of Pseudomonas fluorescens @ 20
    g/m2 at sowing for the management of rice root-knot nematode,      Meloidogyne graminicola in direct
    seeded rice.

    Pulses:
    1.Management of root-knot nematode, 
    Meloidogyne incognita  in
    mung:     Seed treatment 
    with Neem Seed Kernal Powder  @ 5
    g/kg and     Trichoderma  viride @ 5
    g/kg separately for the management of root-knot nematode,     Meloidogyne
    incognita     in mung.
    Fiber crops(Jute) :
    Management of disease complex of Jute caused by   Meloidogyne incognita  and Ralstonia
    solanacearum:     Application of
    Carbofuran @ 1 kg a.i./ha and Biofor pf     
    @ 2.50 kg/ha (with vermicompost at 1:10) one day before sowing of seeds
    for the management of root-knot nematode,     
    Meloidogyne incognita and Ralstonia solanacearum in jute.
    Citrus:
    Management of citrus nematode, Tylenchulus semipenetrans
    using bioagent:     Apply 200 g Trichoderma viride per plant
    (enriched with vermicompost/FYM) for management of citrus nematode,     Tylenchulus semipenetrans.
    ( Procedure for enrichment:20 kg Trichoderma viride mixed with 180 kg of
    Vermicompost / well decomposed FYM. This mixture should be covered with gunny
    bag and to be placed in shade for 15 days. Periodical sprinkling with water to
    be done in order to maintain adequate humidity. 200 g of this mixture     should be applied per plant around the base twice in
    a year, along with application of fertilizer.)

  • · 
    Avoidable
    loss due to Meloidogyne graminicolain
    rice is 16.52 -20.60 %



    · 
    Avoidable
    loss due to Hirschmanniella oryzae in
    rice is 10.23 - 13.08%



    Avoidable loss due toMeloidogyne incognita Race-2in Tomato, Brinjal, Mung, Chilli,
    French Bean, Okra, Cucumber, Blackgram, Cowpea, Bitter Gourd, Pea and Chick Pea
    is 12.86-13.2%, 15.56-16.65%, 23.33 – 26.57%, 14.75%,  17.62-19.8%, 17.6-21.19%, 15.65-17.92%,
    15.85%, 17.78%, 15.38%, 16.0% and 14.53% respectively
  • A
    total of 321 germplasm of rice was screened in
    vitro     against Meloidogyne
    graminicola     during 2012 - 2023, out of which 08 germplasm ((IVT-2016-4,
    IVT-2016-6, IVT-2016-9, IVT-2016-14, IVT-2016-17, IVT-2016-19, IVT-2016-38,
    IVT-2016-42)     were recorded resistant and 38
    germplasm were recorded as moderately resistant ((AV-1-13-3702, AV-1-13-3708, AV-1-13-3710,
    AV-1-13-3717, AV-1-13-3719, AV-1-13-3722, AV-1-13-3725,     A1-14-04, A2-14-25, A2-14-28, GM-14-36, GM-14-37 , AB104, AB 108, IVT-2016-5, IVT-2016-8, IVT-2016-10,
    IVT-2016-11, IVT-2016-12, IVT-2016-13, IVT-2016-15, IVT-2016-16, IVT-2016-18,
    IVT-2016-20, IVT-2016-21, IVT-2016-25, IVT-2016-27, IVT-2016-28,  IVT-2016-29, IVT-2016-30, IVT-2016-31,
    IVT-2016-32, IVT-2016-33, IVT-2016-34, IVT-2016-35, IVT-2016-39, IVT-2016-40,
    IVT-2016-41, IVT-2016-44)      to M. graminicola.
  • 1. Pseudomonus flourescens enriched FYM @ 500 kg/ha (1.0 kg Pf in 100 kg FYM incubated for 15 days) + soil
    application of carbofuran@ 1.0 kg a.i./ha at 45 DAS) was found  effective in reducing population of Meloidogyne graminicola and increasing
    yield of direct seeded  rice
    2. In
    transplanted rice, maximum grain yield (3.715 t/ha), minimum soil and root
    population and RKI of     M. graminicola
    were recorded when carbofuran was applied @ 1.0 kg a.i./ha at sowing
    3. The
    combine application of neem cake @100g/m2 and carbofuran @1.0kg a.i./ha after
    45 days of sowing was effective in reducing the population of     M. graminicola and increasing yield in
    direct seeded ahu rice
    4. Trichoderma viride @10g/kg seed as seed treatment and  soil application @2.5 kg/ha at 45 DAS was
    found to be most effective in reducing population of M. graminicola(52.25%)  and increasing yield (25.23%) with ICBR
    1:5.25
    5. Soil application of Pseudomonas fluorescens @ 20g/m2 and Bacillus subtilis @20g/m2at sowing were found effective against M. graminicola in direct seeded rice
    6. Nursery treatment with Biofor pf-2 @20g/m2 was found effective in reducing
    population of     M.   graminicola and increasing
    yield


  • 135
    germplasm of Brinjal and 100 germplasm of Okra were screened against Meloidogyne incognita in controlled
    condition. One each of brinjal (IC
    112315)    and okra germplasm (EC
    306700)     was recorded as resistant
    while, three and two of brinjal and okra respectively were recorded as
    moderately resistant.
  • 1. Application
    of Purpureocillium lilacinum@ 20g/m2(cfu
    2x106) + neem cake @100g/m2 was found  effective against M .incognita in cowpea, where the yield was 51 q/ha as against 39
    q/ha in untreated control
    2. Seed
    treatment with     Purpureocilliumlilacinum
    @ 2.5 ml/kg +     Pochonia chlamydosporia
    @ 2.5 ml/kg followed by soil application of vermicompost @ 2.5t/ha      enriched with P. chlamydosporia and P.
    lilacinum e    ach @ 5ml/kg was found effective against M. incognita in okra where the yield was 8.81 t/ha as against 6.58
    t/ha in untreated control
    3.Biofumigants,
    cabbage leaves @ 50 t/ha was found effective in reducing population of     M. incognita in okra
    4. Nursery bed treatment with neem cake @ 2 t/ha is found effective in reducing infestation of root-knot nematode in main field and increasing yield 


  • ·       
    Random
    survey was conducted in twelve districts of Assam for occurrence of Meloidogyne graminicola and Hirschmanniella oryzae.



                Shillongoni
    (26021.942ʹ; 092038.447ʹ), Kuruabahi (26018.603ʹ;
    092036.868ʹ), Bhalukamari (26017.173ʹ; 092033.054ʹ)
    Burhapahar (26.572032, 93.143783), Lanka (25.926938;92.955365
    & 25.928298; 92.957468), Bechamari (26.407948; 92.47003)of Nagaon district;
    Areajhar (26015.291ʹ; 090024.133ʹ), Andutzar (26025.112ʹ;
    090025.262ʹ), Kajaikata (26011.134ʹ; 90010.478ʹ)
    of Dhuburi district; Nayagaon (26010.284ʹ; 90011.04211)
    of Bongaigaon district wewre recorded as hot spot area for M. graminicola.



                Ahom gaon(26.398686; 92.479761), Kuruabahi 26018.603ʹ
    E-092036.868ʹ) of Nagaon district; Tarajuli
    of Sonitpur (    26051.105ʹ; 92038.023ʹ) was
    identified as hot spot of Hirschmanniella oryzae

  • ·       
    Four deep water rice growing districts of Assam
    were surveyed during 2012-22  for
    occurrence of ufra disease



                Rantijan
    (270 07.578ʹ; 94007.989ʹ) and Dolpata (27005.430ʹ;
    94006.854ʹ) of Lakhimpur and Nanadi bhakatgaon (27025.109ʹ;
    94023.281ʹ), Rangajan (27o25.098ʹ; 94o
    23.268    ʹ) of Dhemaji district were identified as hot spots of Ditylenchus angustus. However, from 2019 onwards infestation of Ditylenchus
    angustus     in these district become drastically lo

  • ·       
    Random
    survey was conducted in eleven districts of Assam for occurrence of Meloidogyne incognita in vegetable crops    .



                Panibhoral(26041.932ʹ;
    93008.976ʹ)and Dagaon (26039.733ʹ; 93009.766ʹ)of
    Sonitpur district;  Bogolijan (27011.922ʹ;
    94003.049ʹ), Nalkata (27014.772ʹ; 94004.817ʹ)
    and Gaurisali(27014.869ʹ; 94003.383) of Lakhimpur
    district; Satber (26014.589ʹ; 90014.362), Surajkhata (26023.434ʹ;
    0900 22.065ʹ), Gutipara (26010.284ʹ 090011.042ʹ),
    Puran khambari (26.079197, 89.971010)  and Hakama (26031.951ʹ; 92055.803ʹ)
    of Dhuburi district; Danichapori (26044.677ʹ; 093056.445ʹ),
    Nagajurigaon (26.6797; 93.4035), Kharua (26.4916; 93.1932) Anandapur (26.7075;
    93.2085) of Golaghat district;Goroimari,
    Bihdiajajikona, Kamalpur, Karipara, Bamungaon, Ahatguri, Ratangaon, Patgaon     of Kamrup district; Charigaon (26o47.417ʹ; 094O14.494ʹ),Upardeori
    (26049.339ʹ;940 6.707ʹ),Namdeori(26048.199ʹ;940 5.818ʹ)Allengmora (26049.246ʹE09401479ʹ)  and Morangaon (26061.708ʹ; 94018.912ʹ)
    of Jorhat district; Puranigudam (26.366064; 92.785984), Ambagan
    (26.51774;92.85436); Lanka (25.928424; 92.95504); Simaluguri (26.362577;
    92.643992) of Nagaon district were identified as hot spots of M. incognita in vegetables

  • Random
    survey was conducted in three pulse growing districts of Assam for the
    occurrence of Meloidogyne incognita    .



                Satber
    (26014.589ʹ; 90014.362) in Dhuburi district; Morangaon
    (26061.708ʹ; 94018.912ʹ) of Jorhat district; Niz
    Kothiatoli (26.211786; 92.725256), Uttar Chang
    Chaki (26.162369; 92.671628) of Nagaon district was
    identified as hot spot area     of M.
    incognita     in pulses

  • ·       
    Random
    survey was conducted in five Jute growing districts of Assam.



                Dagaon
    (26039.733ʹ; 93009.766ʹ) of Sonitpur district, Tokoubari
    (26021.601ʹ; 92037.717ʹ), Lochanabari (26016.023ʹ;
    92024.139ʹ) of Nagaon District; Gutipara(26010.284ʹ;90011.042ʹ),
    Islamnagar (26012.564ʹ; 90013.554ʹ) of Dhuburi district
    were recorded as hot spot area of M. incognita

  • ·       
    Random
    survey was conducted in eleven districts of Assam for the occurrence of plant
    parasitic nematodes in fruit crops (banana, citrus, guava, papaya)    .



    ·       
    Major plant parasitic nematodes recorded around the
    rhizosphere of different fruit crops were: Meloidogyne
    incognita, Helicotylenchusdihystera, Hoplolaimus indicus,
    Tylenchorhynchusleviterminalis, Macroposthonia    spp.



    Trichoidorusborai was recorded for the first time around the
    rhizosphere of banana 
  • Steinernema abbasi, Oscheius spp, Heterorhabditis bacteriophora, Steinernema
    ceratophorum, Steinernema ceratophorum, Steinernema tami, Steinernema     spp. were isolated and identified from different districts of Assam
  • Study
    on the host races of M. graminicola
    revealed that based on     Rf value, rice (PB1121and Century Patna), weeds (Dactyloctenium aegyptium and Leptochloa
    chinensis    ) were found as host of M.
    graminicola
  • 200,
    195, 210,81, 5, 4, 68 and 80 germplasm of blackgram, green gram, lentil, field
    pea, rajmah, lathyrus, pigeon pea and chickpea, respectively  were screened against M. incognita under controlled condition. One of greengram, 8 of
    lentill, 4 of field pea were recorded resistant against the target nematode
  •  1. Meloidogyne incognita in mung bean can be
    effectively managed by following     mung – toria – paddy or Black gram-potato-maize rotation. However, in
    blackgram as main crop the sequence black gram – cabbage – okra is economically
    good
    2. soil application of neem cake @1t/ha + seed treatment with B.subtilis @10g/kg seed was
    found to be effectiv    e in reducing the infestation of Meloidogyne incognita and increasing yield of mung bean
    3. soil application of neem cake @1t/ha + seed treatment with B.subtilis @10g/kg seed was found to be effective in reducing the infestation of Meloidogyne incognita and increasing yield of cow pe
  • Disease complex of Jute (M.incognita
    and Ralstonia
    solanacearum    ) could be managed by
    soil application of Trichoderma
    viride     @ 2.5 kg/ha at sowing the
    seeds and can obtain more fibre yield (35.51%)
  • Trichoderma viride @20g/m2 was effective in declining the
    population of Tylenchulus semipenetrans
    in Assam lemon where 44.82 ton/ha yield was recorded, which was an increase of
    20.38% over control
  • 1. Cucumber in protective cultivation can be protected from Meloidogyne incognita using bio control agents. Spot application
    (SA) of     Bacillus pumilus @ 1kg/ polyhouse (1000 m2) at the time of planting is effective in managing RKN



    2. Capsicum in protective cultivation can be protected from Meloidogyne incognita by Spot application of G. fasciculatum@ 1kg before planting + SA of Pochonia chlamydosporia @ 1kg/ polyhouse (1000 m2) at 45 DAP


aicrp.nematode[at]aau.ac.in